Anti-EEF1A1 (316-328) antibody produced in rabbit

Name	Anti-EEF1A1 (316-328) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	E3909
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 316-328 of human EEF1A1
Description	Rabbit Polyclonal
Gene	EEF1A1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-EEF1A1 (316-328), <B>Cat. No. E3909</B>: Immunofluorescence of HUVEC cells using Anti-EEF1A1 (316-328), E3909 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-EEF1A1 (316-328), Cat. No. E3909: Immunofluorescence of HUVEC cells using Anti-EEF1A1 (316-328), E3909 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Cell lysates of HEK-293T was separated on SDS-PAGE, blotted with Anti-EEF1A1 (316-328) (<B>Cat. No. E3909</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500

Immunoblotting
Cell lysates of HEK-293T was separated on SDS-PAGE, blotted with Anti-EEF1A1 (316-328) (Cat. No. E3909) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500

<B>Immunofluorescence Graph</B><BR/>Anti-EEF1A1 (316-328), <B>Cat. No. E3909</B>: Intensity analysis of Anti-EEF1A1 (316-328) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different Antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Immunofluorescence Graph
Anti-EEF1A1 (316-328), Cat. No. E3909: Intensity analysis of Anti-EEF1A1 (316-328) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different Antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Assignment of human elongation factor 1alpha genes: EEF1A maps to chromosome 6q14 - Assignment of human elongation factor 1alpha genes: EEF1A maps to chromosome 6q14

Lund A, Knudsen SM, Vissing H, Clark B, Tommerup N. Genomics. 1996 Sep 1;36(2):359-61.

Eukaryotic translation elongation factor 1A induces anoikis by triggering cell - Eukaryotic translation elongation factor 1A induces anoikis by triggering cell

Itagaki K, Naito T, Iwakiri R, Haga M, Miura S, Saito Y, Owaki T, Kamiya S, Iyoda T, Yajima H, Iwashita S, Ejiri S, Fukai F. J Biol Chem. 2012 May 4;287(19):16037-46.

Association of translation factor eEF1A with defective ribosomal products - Association of translation factor eEF1A with defective ribosomal products

Meriin AB, Zaarur N, Sherman MY. J Cell Sci. 2012 Jun 1;125(Pt 11):2665-74.

[Effect of knocking down eEF1A1 gene on proliferation and apoptosis in Jurkat - [Effect of knocking down eEF1A1 gene on proliferation and apoptosis in Jurkat

Huang Y, Hu JD, Qi YL, Wu YA, Zheng J, Chen YY, Huang XL. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Aug;20(4):835-41.

iTRAQ identification of candidate serum biomarkers associated with metastatic - iTRAQ identification of candidate serum biomarkers associated with metastatic

Rehman I, Evans CA, Glen A, Cross SS, Eaton CL, Down J, Pesce G, Phillips JT, Yen OS, Thalmann GN, Wright PC, Hamdy FC. PLoS One. 2012;7(2):e30885.