ImmunofluorescenceHeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton
™ X-100. Fixed cells were stained with 2 μg Rabbit Anti-COG4 (
Cat. No. SAB4200568). The antibody was developed using Goat Anti-Rabbit IgG, Cy3
™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingCell line lysates were separated on SDS-PAGE and probed with 5 μg/mL Rabbit Anti-COG4 (
Cat. No. SAB4200568). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. HeLa
2. HepG2
3. CHO
4. NIH-3T3
5. Neuro-2a
6. NRK
7. Hepa1-6
8. Rat liver