ImmunofluorescenceHeLa cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Mouse Anti-BTN1A1 Clone: BTN1A1-A116 (
Cat. No. SAB4100132) diluted to 1:200. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
ImmunoblottingLysate of HEK-293T cells expressing FLAG
®-tagged human BTN1A1 was separated on SDS-PAGE, probed with Mouse Anti-BTN1A1 Clone: BTN1A1-A116 (
Cat. No. SAB4100132). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:2,000
2. Negative control
3. Positive control Anti-FLAG
®
ImmunoblottingHeLa cell extract was separated on SDS-PAGE and probed with Mouse Anti-BTN1A1 Clone: BTN1A1-A116 (
Cat. No. SAB4100132). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:250
2. Antibody dilution 1:500
3. Negative control