Anti-PPM1M (C-terminal) antibody produced in rabbit

Name	Anti-PPM1M (C-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200169
Prices	$55.60, $322.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 261-279 of human PPM1M conjugated KLH
Description	Rabbit Polyclonal
Gene	PPM1M
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells over-expressing hPPM1M was separated on SDS-PAGE and probed with Rabbit Anti-hPPM1M (<B>Cat. No. SAB4200169</B>). The antibody was developed with Goat anti-Rabbit IgG, Peroxidase conjugate (<B>Cat. No. A0545</B>) and a chemiluminescence substrate. <BR/><B>Lanes</B><BR/>1. Antibody 8 μg/mL<BR/>2. Antibody 4 μg/mL<BR/>3. Antibody 2 μg/mL<BR/>4. Antibody 4 μg/mL + 10 μg/mL hPPM1M immunizing peptide

Immunoblotting
Lysate of HEK-293T cells over-expressing hPPM1M was separated on SDS-PAGE and probed with Rabbit Anti-hPPM1M (Cat. No. SAB4200169). The antibody was developed with Goat anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate.
Lanes
1. Antibody 8 μg/mL
2. Antibody 4 μg/mL
3. Antibody 2 μg/mL
4. Antibody 4 μg/mL + 10 μg/mL hPPM1M immunizing peptide

<B>Immunoprecipitation</B><BR/>Rabbit Anti-hPPM1M (<B>Cat. No. SAB4200169</B>) was used to immunoprecipitate hPPM1M from lysate of HEK-293T cells over-expressing hPPM1M. <BR/><B>Lanes</B><BR/>1. IP antibody: 10 μg<BR/>2. IP antibody: 5 μg<BR/>3. IP antibody: 2.5 μg<BR/>4. Negative control: without IP antibody<BR/>5. Negative control: without lysate

Immunoprecipitation
Rabbit Anti-hPPM1M (Cat. No. SAB4200169) was used to immunoprecipitate hPPM1M from lysate of HEK-293T cells over-expressing hPPM1M.
Lanes
1. IP antibody: 10 μg
2. IP antibody: 5 μg
3. IP antibody: 2.5 μg
4. Negative control: without IP antibody
5. Negative control: without lysate

Product References

A mechanism for the suppression of interleukin-1-induced nuclear factor kappaB - A mechanism for the suppression of interleukin-1-induced nuclear factor kappaB

Henmi T, Amano K, Nagaura Y, Matsumoto K, Echigo S, Tamura S, Kobayashi T. Biochem J. 2009 Sep 14;423(1):71-8.

Molecular cloning of PP2Ceta, a novel member of the protein phosphatase 2C - Molecular cloning of PP2Ceta, a novel member of the protein phosphatase 2C

Komaki K, Katsura K, Ohnishi M, Guang Li M, Sasaki M, Watanabe M, Kobayashi T, Tamura S. Biochim Biophys Acta. 2003 Nov 30;1630(2-3):130-7.