ImmunofluorescenceAnti-RPL7A (226-240):
Cat. No. R1657: Immunofluorescence of HUVEC cells using RPL7A (226-240) (RB),
Cat. No. R1657 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
ImmunoblottingHEK-293T cell lysate was separated on SDS-PAGE, blotted with Anti-RPL7A (226-240) (
Cat. No. R1657) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
ChartAnti-RPL7A (226-240):
Cat. No. R1657: Intensity analysis of Anti-RPL7A (226-240) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.