Anti-COG8 (C-terminal) antibody produced in rabbit

Name	Anti-COG8 (C-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200427
Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to the C-terminal region of human COG8, conjugated to KLH
Description	Rabbit Polyclonal
Gene	COG8
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 1% paraformaldehyde in a buffer containing 10 mM MES pH 6.2, 10 mM NaCl, 1.5 mM MgCl<SUB>2</SUB> and 2.5% glycerol and subsequently permeabilized with 0.1% Triton<SUP>™</SUP> X-100. Fixed cells were co-immunostained with 1 μg Rabbit Anti-COG8 (C-terminal) (<B>Cat. No. SAB4200427</B>) followed by Goat Anti-Rabbit IgG, Cy3<SUP>™</SUP> conjugate (red, panel A and B) and with a Golgi marker (green, panel B). Cells were counterstained with DAPI to stain nuclei (blue, panel B). The pictures are courtesy of Dr. Orly Laufman and Prof. Sima Lev, Dept. of Molecular Cell Biology, Weizmann institute of Science, Rehovot, Israel.

Immunofluorescence
HeLa cells were fixed and permeabilized with 1% paraformaldehyde in a buffer containing 10 mM MES pH 6.2, 10 mM NaCl, 1.5 mM MgCl₂ and 2.5% glycerol and subsequently permeabilized with 0.1% Triton^™ X-100. Fixed cells were co-immunostained with 1 μg Rabbit Anti-COG8 (C-terminal) (Cat. No. SAB4200427) followed by Goat Anti-Rabbit IgG, Cy3^™ conjugate (red, panel A and B) and with a Golgi marker (green, panel B). Cells were counterstained with DAPI to stain nuclei (blue, panel B). The pictures are courtesy of Dr. Orly Laufman and Prof. Sima Lev, Dept. of Molecular Cell Biology, Weizmann institute of Science, Rehovot, Israel.

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X100. Fixed cells were stained with 1 μg/mL Rabbit Anti-COG8 (C-terminal) (<B>Cat. No. SAB4200427</B>). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X100. Fixed cells were stained with 1 μg/mL Rabbit Anti-COG8 (C-terminal) (Cat. No. SAB4200427). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Cell line lysates were separated on SDS-PAGE and probed with 2.5 μg/mL Rabbit Anti-COG8 (C-terminal) (<B>Cat. No. SAB4200427</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. HEK-293T; 2. HeLa; 3. JURKAT; 4. MCF-7; 5. MDA-MB-231; 6. A431; 7. SW480

Immunoblotting
Cell line lysates were separated on SDS-PAGE and probed with 2.5 μg/mL Rabbit Anti-COG8 (C-terminal) (Cat. No. SAB4200427). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HEK-293T; 2. HeLa; 3. JURKAT; 4. MCF-7; 5. MDA-MB-231; 6. A431; 7. SW480

<B>Immunoprecipitation </B><BR/>Rabbit Anti-COG8 (C-terminal) (<B>Cat. No. SAB4200427</B>) was used to immunoprecipitate COG8 from HEK-293T. Detection antibody: Rabbit Anti-COG8 (N-terminal) (<B>Cat. No. SAB4200428</B>).<BR/><B>Lanes</B><BR/>1. 5 μg antibody<BR/>2.10 μg antibody<BR/>3. Negative control: 10 μg non-relevant antibody

Immunoprecipitation
Rabbit Anti-COG8 (C-terminal) (Cat. No. SAB4200427) was used to immunoprecipitate COG8 from HEK-293T. Detection antibody: Rabbit Anti-COG8 (N-terminal) (Cat. No. SAB4200428).
Lanes
1. 5 μg antibody
2.10 μg antibody
3. Negative control: 10 μg non-relevant antibody