Monoclonal Anti-Tom22−Atto 488 antibody produced in mouse

Name	Monoclonal Anti-Tom22−Atto 488 antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	T4327
Prices	$413.50
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	IC9-2
Applications	ICC/IF
Species Reactivities	Monkey, Human
Antigen	membrane fraction from Vero (monkey kidney-derived) cells
Description	Mouse Monoclonal
Gene	TOMM22
Conjugate	Atto 488 conjugate
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Co-staining of mitochondria and ER-Golgi intermediate compartment in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were co-stained with 2 μg/mL Mouse Anti-TOM22, Atto 488 Conjugate (<B>Cat. No. T4237</B>) (mitochondria stained in green) and 1 μg/mL Rabbit Anti-ERGIC-53/p58, Cy3 Conjugate (<B>Cat. No. E6782</B>) (ER-Golgi intermediate compartment stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
Co-staining of mitochondria and ER-Golgi intermediate compartment in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were co-stained with 2 μg/mL Mouse Anti-TOM22, Atto 488 Conjugate (Cat. No. T4237) (mitochondria stained in green) and 1 μg/mL Rabbit Anti-ERGIC-53/p58, Cy3 Conjugate (Cat. No. E6782) (ER-Golgi intermediate compartment stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunofluorescence</B><BR/>Co-staining of mitochondria and nuclear envelope in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were co-stained with 2 μg/mL Mouse Anti-TOM22, Atto 488 Conjugate (<B>Cat. No. T4237</B>) (mitochondria stained in green) and 10 μg/mL Rabbit Anti-Lamin A, Atto 647N Conjugate (<B>Cat. No. L3544</B>) (nuclear envelope stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
Co-staining of mitochondria and nuclear envelope in HeLa cells. HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were co-stained with 2 μg/mL Mouse Anti-TOM22, Atto 488 Conjugate (Cat. No. T4237) (mitochondria stained in green) and 10 μg/mL Rabbit Anti-Lamin A, Atto 647N Conjugate (Cat. No. L3544) (nuclear envelope stained in red). Cells were counterstained with DAPI (blue) to stain nuclei.

Product References

Identification of mammalian TOM22 as a subunit of the preprotein translocase of - Identification of mammalian TOM22 as a subunit of the preprotein translocase of

Saeki K, Suzuki H, Tsuneoka M, Maeda M, Iwamoto R, Hasuwa H, Shida S, Takahashi T, Sakaguchi M, Endo T, Miura Y, Mekada E, Mihara K. J Biol Chem. 2000 Oct 13;275(41):31996-2002.