Anti-COL5A2 (1361-1375) antibody produced in rabbit

Name	Anti-COL5A2 (1361-1375) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	C3373
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 1361-1375 of human COL5A2
Description	Rabbit Polyclonal
Gene	COL5A2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-COL5A2 (1361-1375), <B>Cat. No. C3373</B>: Immunofluorescence of HUVEC cells using Anti-COL5A2 (1361-1375), C3373 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-COL5A2 (1361-1375), Cat. No. C3373: Immunofluorescence of HUVEC cells using Anti-COL5A2 (1361-1375), C3373 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human FLAG - COL5A2 fusion protein was separated on SDS-PAGE, blotted with Anti-COL5A2 (1361-1375) (<B>Cat. No. C3373</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + COL5A2 immunizing peptide (human,1361-1375)<BR/>3. Antibody dilution 1:1,000 <BR/>4. Negative control (only secondary antibody)<BR/>5. Anti-FLAG antibody (<B>Cat. No. F7425</B>), working concentration 2 μg/mL

Immunoblotting
Lysate of HEK-293T cells overexpressing human FLAG - COL5A2 fusion protein was separated on SDS-PAGE, blotted with Anti-COL5A2 (1361-1375) (Cat. No. C3373) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + COL5A2 immunizing peptide (human,1361-1375)
3. Antibody dilution 1:1,000
4. Negative control (only secondary antibody)
5. Anti-FLAG antibody (Cat. No. F7425), working concentration 2 μg/mL

<B>Immunofluorescence Graph</B><BR/>Anti-COL5A2 (1361-1375), <B>Cat. No. C3373</B>: Intensity analysis of Anti-COL5A2 (1361-1375) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different Antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Immunofluorescence Graph
Anti-COL5A2 (1361-1375), Cat. No. C3373: Intensity analysis of Anti-COL5A2 (1361-1375) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different Antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.