Western blot analysis of nestin phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) mouse embryonic brain tissue extract. Antibodies tested include p-nestin (Thr 1495): sc-33880 (A,B) and nestin (C-20): sc-21247 (C,D).
Western blot analysis of nestin phosphorylation in untreated (A,D), induction cocktail (sc-362324) treated (B,E) and induction cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) SH-SY5Y whole cell lysates. Antibodies tested include p-nestin (Thr 1495): sc-33880 (A,B,C) and nestin (C-20): sc-21247 (D,E,F).