ImmunofluorescenceAnti-PPFIA3 (701-715):
Cat. No. F3306: Immunofluorescence of HUVEC cells using PPFIA3 (701-715),
Cat. No. F3306 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
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ImmunoblottingLysate of HEK-293T cells overexpressing human PPFIA3 was separated on SDS-PAGE, blotted with Anti-PPFIA3 (701-715) (
Cat. No. F3306) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:1,000
ChartAnti-PPFIA3 (701-715):
Cat. No. F3306: Intensity analysis of Anti-PPFIA3 (701-715) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.