ImmunofluorescenceHeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 5 μg/mL Rabbit Anti-NMD3 (
Cat. No. SAB4200451). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingWhole extract of HEK-293T cells overexpressing human NMD3 was separated on SDS-PAGE and probed with Rabbit Anti-NMD3 (
Cat. No. SAB4200451). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. 1 μg/mL antibody
2. 2 μg/mL antibody
3. 5 μg/mL antibody