ImmunoblottingWhole extract of HEK-293T cells overexpressing human COG6 was separated on SDS-PAGE and probed with Rabbit Anti-COG6 (
Cat. No. SAB4200441). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 0.5 μg/mL antibody
2. 1 μg/mL antibody
3. 1 μg/mL antibody + COG6 immunizing peptide
ImmunoprecipitationRabbit Anti-COG6 (
Cat. No. SAB4200441) was used to immunoprecipitate COG6 from HeLa whole cell extract. Detection antibody: Rabbit Anti-COG6 (C-terminal) (
Cat. No. SAB4200442).
Lanes
1. 10 μg antibody
2. 20 μg antibody
3. Negative control: 10 μg non-relevant antibody
4. Positive control: HeLa whole cell extract