Monoclonal Anti-BTBD9 antibody produced in mouse

Name	Monoclonal Anti-BTBD9 antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4200447
Prices	$337.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Clone	BTBD9-205
Applications	ICC/IF WB
Species Reactivities	Human, Monkey, Dog, Mouse
Antigen	a peptide corresponding to a sequence at the C-terminus of human BTBD9, conjugated to KLH
Description	Mouse Monoclonal
Gene	BTBD9
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-BTBD9 Clone: BTBD9-205 (<B>Cat. No. SAB4200447</B>) diluted 1:1,000. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-BTBD9 Clone: BTBD9-205 (Cat. No. SAB4200447) diluted 1:1,000. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>K562 cell extract was separated on SDS-PAGE and probed with Mouse Anti-BTBD9 Clone: BTBD9-205 (<B>Cat. No. SAB4200447</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:16,000<BR/>2. Antibody dilution 1:16,000 + 60 μg/mL BTBD9 (Internal) immunizing peptide<BR/>3. Antibody dilution 1:16,000 + 60 μg/mL BTBD9 (C-ter) immunizing peptide

Immunoblotting
K562 cell extract was separated on SDS-PAGE and probed with Mouse Anti-BTBD9 Clone: BTBD9-205 (Cat. No. SAB4200447). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:16,000
2. Antibody dilution 1:16,000 + 60 μg/mL BTBD9 (Internal) immunizing peptide
3. Antibody dilution 1:16,000 + 60 μg/mL BTBD9 (C-ter) immunizing peptide

<B>Immunoblotting</B><BR/>Lysates were separated on SDS-PAGE and probed with antibody dilution 1:10,000 Mouse Anti-BTBD9 Clone: BTBD9-205 (<B>Cat. No. SAB4200447</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. HeLa; 2. A431; 3. 3T3; 4. Hepa 1-6; 5. L8; 6. PC12; 7. COS-7; 8. MDCK

Immunoblotting
Lysates were separated on SDS-PAGE and probed with antibody dilution 1:10,000 Mouse Anti-BTBD9 Clone: BTBD9-205 (Cat. No. SAB4200447). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. HeLa; 2. A431; 3. 3T3; 4. Hepa 1-6; 5. L8; 6. PC12; 7. COS-7; 8. MDCK