ImmunofluorescenceAnti-ENOPH1 (181-195) (RB):
Cat. No. E8532: Immunofluorescence of HUVEC cells using ENOPH1 (181-195) (RB),
Cat. No. E8532 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
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ImmunoblottingLysate of HEK-293T cells overexpressing human ENOPH1 was separated on SDS-PAGE, blotted with Anti-ENOPH1 (181-195) (
Cat. No. E8532) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:1,000
2. Antibody dilution 1:1,000 + ENOPH1 immunizing peptide (human, 181-195)
ChartAnti-ENOPH1 (181-195) (RB):
Cat. No. E8532: Intensity analysis of Anti-ENOPH1 (181-195) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.