ImmunofluorescenceAnti-TM4SF4 (126-140) (RB):
Cat. No. SAB1100990: Immunofluorescence of HUVEC cells using Anti-TM4SF4 (126-140) (RB) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
Immunofluorescence GraphAnti-TM4SF4 (126-140) (RB):
Cat. No. SAB1100990: Intensity analysis of Anti-TM4SF4 (126-140) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6400) as compared with the negative control.
ImmunoblottingLysate of HEK-293T cells overexpressing human TM4SF4 was separated on SDS-PAGE, blotted with Anti-TM4SF4 (126-140) (
Cat. No. SAB1100990) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
2. Antibody dilution 1:500 + TM4SF4 immunizing peptide (human, 126-140)