Anti-AK7 (296-310) antibody produced in rabbit

Name	Anti-AK7 (296-310) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	A4482
Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 296-310 of human AK7
Description	Rabbit Polyclonal
Gene	AK7
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-AK7 (296-310) : <B>Cat. No. A4482</B>: Immunofluorescence of HUVEC cells using Anti-AK7 (296-310) (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.

Immunofluorescence
Anti-AK7 (296-310) : Cat. No. A4482: Immunofluorescence of HUVEC cells using Anti-AK7 (296-310) (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.

<B>Immunofluorescence Graph</B><BR/>Anti-AK7 (296-310) : <B>Cat. No. A4482</B>: Intensity analysis of Anti-AK7 (296-310) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Immunofluorescence Graph
Anti-AK7 (296-310) : Cat. No. A4482: Intensity analysis of Anti-AK7 (296-310) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human AK7 was separated on SDS-PAGE, blotted with Anti-AK7 (296-310) (<B>Cat. No. A4482</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + AK7 immunizing peptide (human, 296-310)<BR/>3. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human AK7 was separated on SDS-PAGE, blotted with Anti-AK7 (296-310) (Cat. No. A4482) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + AK7 immunizing peptide (human, 296-310)
3. Antibody dilution 1:1,000

Product References

Role of adenylate kinase type 7 expression on cilia motility: possible link in - Role of adenylate kinase type 7 expression on cilia motility: possible link in

Milara J, Armengot M, Mata M, Morcillo EJ, Cortijo J. Am J Rhinol Allergy. 2010 May-Jun;24(3):181-5.

New adenylate kinase 7 (AK7) mutation in primary ciliary dyskinesia. - New adenylate kinase 7 (AK7) mutation in primary ciliary dyskinesia.

Mata M, Lluch-Estelles J, Armengot M, Sarrion I, Carda C, Cortijo J. Am J Rhinol Allergy. 2012 Jul-Aug;26(4):260-4.