ImmunoblottingLysate of HEK-293T cells was separated on SDS-PAGE, blotted with Anti-SPZ1 (351-365) (
Cat. No. SAB1103090) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
ImmunoblottingLysate of HEK-293T cells overexpressing human SPZ1 was separated on SDS-PAGE, blotted with Anti-SPZ1 (351-365) (
Cat. No. SAB1103090) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:1,000
2. Antibody dilution 1:1,000 + SPZ1 immunizing peptide (human, 351-365)