Anti-SORBS2 (N-terminal) antibody produced in rabbit

Name	Anti-SORBS2 (N-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200038
Prices	$61.10, $360.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 29-46 of human SORBS2 isoform 2, conjugated to KLH
Description	Rabbit Polyclonal
Gene	SORBS2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Cytoplasmic localization of SORBS2 in HeLa cells. Fixed cells were stained with 20 μg/mL Anti-SORBS2 (N-terminal) (<B>Cat. No. SAB4200038</B>) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.

Immunofluorescence
Cytoplasmic localization of SORBS2 in HeLa cells. Fixed cells were stained with 20 μg/mL Anti-SORBS2 (N-terminal) (Cat. No. SAB4200038) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.

<B>Immunoblotting</B><BR/>Lysates of HEK-293T cells overexpressing human SORBS2 (lanes 1,2), HEK-293T non transfected (lane 3), HeLa cells (lanes 4,6) and A431 cells (lane 5) were separated on SDS-PAGE, blotted with Anti-SORBS2 (N-terminal) (<B>Cat. No. SAB200038</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody: 0.15 μg/mL<BR/>2. Antibody: 0.15 μg/mL + SORBS2 immunizing peptide (human, 29-46)<BR/>3. Antibody: 0.15 μg/mL<BR/>4. Antibody: 0.75 μg/mL<BR/>5. Antibody: 0.75 μg/mL<BR/>6. Antibody: 0.75 μg/mL + SORBS2 immunizing peptide (human, 29-46)

Immunoblotting
Lysates of HEK-293T cells overexpressing human SORBS2 (lanes 1,2), HEK-293T non transfected (lane 3), HeLa cells (lanes 4,6) and A431 cells (lane 5) were separated on SDS-PAGE, blotted with Anti-SORBS2 (N-terminal) (Cat. No. SAB200038) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate.
Lanes
1. Antibody: 0.15 μg/mL
2. Antibody: 0.15 μg/mL + SORBS2 immunizing peptide (human, 29-46)
3. Antibody: 0.15 μg/mL
4. Antibody: 0.75 μg/mL
5. Antibody: 0.75 μg/mL
6. Antibody: 0.75 μg/mL + SORBS2 immunizing peptide (human, 29-46)

Product References

ArgBP2, a multiple Src homology 3 domain-containing, Arg/Abl-interacting protein, - ArgBP2, a multiple Src homology 3 domain-containing, Arg/Abl-interacting protein,

Wang B, Golemis EA, Kruh GD. J Biol Chem. 1997 Jul 11;272(28):17542-50.

Cbl-ArgBP2 complex mediates ubiquitination and degradation of c-Abl. - Cbl-ArgBP2 complex mediates ubiquitination and degradation of c-Abl.

Soubeyran P, Barac A, Szymkiewicz I, Dikic I. Biochem J. 2003 Feb 15;370(Pt 1):29-34.

ArgBP2 and the SoHo family of adapter proteins in oncogenic diseases. - ArgBP2 and the SoHo family of adapter proteins in oncogenic diseases.

Roignot J, Soubeyran P. Cell Adh Migr. 2009 Apr-Jun;3(2):167-70. Epub 2009 Apr 5.