ImmunofluorescenceFocal adhesions and nuclear localization of tensin 1 in chicken embryo fibroblast cells. Fixed cells were stained with 2 μg/mL Anti-Tensin 1 (C-terminal region) (
Cat. No. SAB4200297) followed by Goat anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
ImmunoblottingTissue and cell extracts were separated on SDS-PAGE, blotted with 1 μg/mL Anti-Tensin 1 ( C-terminal region) (
Cat. No. SAB4200297), and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate. Additional lower bands observed in rat heart and A10 cells may represent tensin1 degradation products.
Lanes1. Rat heart
2. A10
3. HEK293-T overexpressing human tensin 1
4. HEK293-T non-transfected
5. HEK293-T overexpressing human tensin 1. Antibody + immunizing tensin 1 peptide
6. Rat heart. Antibody + immunizing tensin 1 peptide