Anti-RER1 antibody produced in rabbit

Name	Anti-RER1 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	R4407
Prices	$62.60, $361.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	ICC/IF WB
Species Reactivities	Rat, Human, Mouse
Antigen	synthetic peptide corresponding to amino acids 181-196 of human Rer1, conjugated to KLH
Description	Rabbit Polyclonal
Gene	RER1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>3T3 cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 1 μg/mL Rabbit Anti-RER1 (<B>Cat. No. R4407</B>). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
3T3 cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 1 μg/mL Rabbit Anti-RER1 (Cat. No. R4407). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunofluorescence</B><BR/>NRK cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 1 μg/mL Rabbit Anti-RER1 (<B>Cat. No. R4407</B>). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
NRK cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 1 μg/mL Rabbit Anti-RER1 (Cat. No. R4407). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 2 μg/mL Rabbit Anti-RER1 (<B>Cat. No. R4407</B>). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Immunofluorescence
HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone (1:1). Fixed cells were stained with 2 μg/mL Rabbit Anti-RER1 (Cat. No. R4407). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

<B>Immunoblotting</B><BR/>Whole extract of HEK-293T cells overexpressing human RER1 was separated on SDS-PAGE and probed with Rabbit Anti-RER1 (<B>Cat. No. R4407</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 2 μg/mL antibody <BR/>2. 5 μg/mL antibody

Immunoblotting
Whole extract of HEK-293T cells overexpressing human RER1 was separated on SDS-PAGE and probed with Rabbit Anti-RER1 (Cat. No. R4407). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 5 μg/mL antibody

Product References

Retention in endoplasmic reticulum 1 (RER1) modulates amyloid-beta (Abeta) - Retention in endoplasmic reticulum 1 (RER1) modulates amyloid-beta (Abeta)

Park HJ, Shabashvili D, Nekorchuk MD, Shyqyriu E, Jung JI, Ladd TB, Moore BD, Felsenstein KM, Golde TE, Kim SH. J Biol Chem. 2012 Nov 23;287(48):40629-40.

Rer1p maintains ciliary length and signaling by regulating gamma-secretase - Rer1p maintains ciliary length and signaling by regulating gamma-secretase

Jurisch-Yaksi N, Rose AJ, Lu H, Raemaekers T, Munck S, Baatsen P, Baert V, Vermeire W, Scales SJ, Verleyen D, Vandepoel R, Tylzanowski P, Yaksi E, de Ravel T, Yost HJ, Froyen G, Arrington CB, Annaert W. J Cell Biol. 2013 Mar 18;200(6):709-20.