Anti-Rab14 (C-terminal) antibody produced in rabbit

Name	Anti-Rab14 (C-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	R0656
Prices	$368.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Rat, Human, Mouse
Antigen	synthetic peptide corresponding to amino acid residues 195-212 of human RAB14 conjugated to KLH
Description	Rabbit Polyclonal
Gene	RAB14
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin. Fixed cells were stained with 5 μg/mL Rabbit Anti-Rab14 (C-terminal) (<B>Cat. No. R0656</B>). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Immunofluorescence
NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin. Fixed cells were stained with 5 μg/mL Rabbit Anti-Rab14 (C-terminal) (Cat. No. R0656). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-Rab14 (C-terminal) (<B>Cat. No. R0656</B>) was used to immunoprecipitate Rab14 from Raji whole cell extract. Detection antibody: Rabbit Anti-Rab14 antibody (<B>Cat. No. R0781</B>). <BR/><B>Lanes</B><BR/>1. 5 μg antibody<BR/>2. Negative control: 5 μg non-relevant antibody<BR/>3. Negative control: without precipitating antibody

Immunoprecipitation
Rabbit Anti-Rab14 (C-terminal) (Cat. No. R0656) was used to immunoprecipitate Rab14 from Raji whole cell extract. Detection antibody: Rabbit Anti-Rab14 antibody (Cat. No. R0781).
Lanes
1. 5 μg antibody
2. Negative control: 5 μg non-relevant antibody
3. Negative control: without precipitating antibody

<B>Immunoblotting</B><BR/>Whole extract of Mouse brain was separated on SDS-PAGE and probed with Rabbit Anti-Rab14 (C-terminal) (<B>Cat. No. R0656</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 2 μg/mL antibody <BR/>2. 2 μg/mL antibody + Rab14 immunizing peptide (human, 195-212)

Immunoblotting
Whole extract of Mouse brain was separated on SDS-PAGE and probed with Rabbit Anti-Rab14 (C-terminal) (Cat. No. R0656). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 2 μg/mL antibody + Rab14 immunizing peptide (human, 195-212)

<B>Immunoblotting</B><BR/>Whole extract of Raji cells was separated on SDS-PAGE and probed with Rabbit Anti-Rab14 (C-terminal) (<B>Cat. No. R0656</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 1 μg/mL antibody <BR/>2. 2 μg/mL antibody<BR/>3. 2 μg/mL antibody + Rab14 immunizing peptide (human, 195-212)

Immunoblotting
Whole extract of Raji cells was separated on SDS-PAGE and probed with Rabbit Anti-Rab14 (C-terminal) (Cat. No. R0656). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 1 μg/mL antibody
2. 2 μg/mL antibody
3. 2 μg/mL antibody + Rab14 immunizing peptide (human, 195-212)