Anti-COL5A2 (266-280) antibody produced in rabbit

Name	Anti-COL5A2 (266-280) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	C3248
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 266-280 of human COL5A2
Description	Rabbit Polyclonal
Gene	COL5A2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human FLAG-COL5A2 fusion protein was separated on SDS-PAGE, blotted with Anti-COL5A2 (266-280) (<B>Cat. No. C3248</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250<BR/>2. Antibody dilution 1:500<BR/>3. Antibody dilution 1:500 + COL5A2 immunizing peptide (human, 266-280)<BR/>4. Antibody dilution 1:1,000<BR/>5. Negative control (only secondary antibody)<BR/>6. Anti-FLAG antibody (<B>Cat. No. F7425</B>), working concentration 2 μg/mL

Immunoblotting
Lysate of HEK-293T cells overexpressing human FLAG-COL5A2 fusion protein was separated on SDS-PAGE, blotted with Anti-COL5A2 (266-280) (Cat. No. C3248) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250
2. Antibody dilution 1:500
3. Antibody dilution 1:500 + COL5A2 immunizing peptide (human, 266-280)
4. Antibody dilution 1:1,000
5. Negative control (only secondary antibody)
6. Anti-FLAG antibody (Cat. No. F7425), working concentration 2 μg/mL

<B>Immunofluorescence</B><BR/>Anti-COL5A2 (266-280): <B>Cat. No. C3248</B>: Immunofluorescence of HUVEC cells using COL5A2 (266-280), Cat. No. C3248 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-COL5A2 (266-280): Cat. No. C3248: Immunofluorescence of HUVEC cells using COL5A2 (266-280), Cat. No. C3248 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Chart</B><BR/>Anti-COL5A2 (266-280): <B>Cat. No. C3248</B>: Intensity analysis of Anti-COL5A2 (266-280) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-COL5A2 (266-280): Cat. No. C3248: Intensity analysis of Anti-COL5A2 (266-280) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.