Anti-PFKFB4 (176-190) antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: F2931
• Prices: $342.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: synthetic peptide corresponding to amino acids 176-190 of human PFKFB4
• Description: Rabbit Polyclonal
• Gene: PFKFB4
• Conjugate: unconjugated

Product images

Immunofluorescence
Anti-PFKFB4 (176-190): Cat. No. F2931: Immunofluorescence of HUVEC cells using PFKFB4 (176-190) (RB),Cat. No. F2931 (red) at a 1:200 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunoblotting
Lysate of HEK-293T cells overexpressing human PFKFB4 was separated on SDS-PAGE, blotted with Anti-PFKFB4 (176-190) (Cat. No. F2931) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000
2. Antibody dilution 1:1,000 + PFKFB4 immunizing peptide (human, 176-190)

Chart
Anti-PFKFB4 (176-190): Cat. No. F2931: Intensity analysis of Anti-PFKFB4 (176-190) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

PFKFB3 activation in cancer cells by the p38/MK2 pathway in response to stress - PFKFB3 activation in cancer cells by the p38/MK2 pathway in response to stress

Novellasdemunt L, Bultot L, Manzano A, Ventura F, Rosa JL, Vertommen D, Rider MH, Navarro-Sabate A, Bartrons R. Biochem J. 2013 Jun 15;452(3):531-43.

Functional metabolic screen identifies - Functional metabolic screen identifies

Ros S, Santos CR, Moco S, Baenke F, Kelly G, Howell M, Zamboni N, Schulze A. Cancer Discov. 2012 Apr;2(4):328-43.

Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation - Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation

Yalcin A, Clem BF, Simmons A, Lane A, Nelson K, Clem AL, Brock E, Siow D, Wattenberg B, Telang S, Chesney J. J Biol Chem. 2009 Sep 4;284(36):24223-32.

Hypoxic regulation of PFKFB-3 and PFKFB-4 gene expression in gastric and - Hypoxic regulation of PFKFB-3 and PFKFB-4 gene expression in gastric and

Bobarykina AY, Minchenko DO, Opentanova IL, Moenner M, Caro J, Esumi H, Minchenko OH. Acta Biochim Pol. 2006;53(4):789-99. Epub 2006 Dec 4.

Hypoxia induces transcription of - Hypoxia induces transcription of

Minchenko O, Opentanova I, Minchenko D, Ogura T, Esumi H. FEBS Lett. 2004 Oct 8;576(1-2):14-20.

Hypoxic regulation of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase - Hypoxic regulation of the 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Minchenko O, Opentanova I, Caro J. FEBS Lett. 2003 Nov 20;554(3):264-70.