Monoclonal Anti-RAVER1 antibody produced in mouse

Name	Monoclonal Anti-RAVER1 antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4200203
Prices	$322.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	RAV1
Applications	IP WB
Species Reactivities	Monkey, Human
Antigen	a peptide corresponding to amino acids 29-45 of human RAVER1
Description	Mouse Monoclonal
Gene	RAVER1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Whole extract of HepG2 cells was separated on SDS-PAGE and probed with Mouse Anti-RAVER1 Clone: RAV1 (<B>Cat. No. SAB4200203</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. 1.0 μg/mL antibody<BR/>2. 1.0 μg/mL antibody + 60 μg/mL RAVER1 (N-ter) immunizing peptide<BR/>3. 1.0 μg/mL antibody + 60 μg/mL RAVER1 (C-ter) immunizing peptide

Immunoblotting
Whole extract of HepG2 cells was separated on SDS-PAGE and probed with Mouse Anti-RAVER1 Clone: RAV1 (Cat. No. SAB4200203). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. 1.0 μg/mL antibody
2. 1.0 μg/mL antibody + 60 μg/mL RAVER1 (N-ter) immunizing peptide
3. 1.0 μg/mL antibody + 60 μg/mL RAVER1 (C-ter) immunizing peptide

<B>Immunoblotting</B><BR/>Lysates were separated on SDS-PAGE and probed with 1.0 μg/mL Mouse Anti-RAVER1 Clone: RAV1 (<B>Cat. No. SAB4200203</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. HeLa<BR/>2. 293T<BR/>3. COS-7

Immunoblotting
Lysates were separated on SDS-PAGE and probed with 1.0 μg/mL Mouse Anti-RAVER1 Clone: RAV1 (Cat. No. SAB4200203). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. HeLa
2. 293T
3. COS-7

<B>Immunoprecipitation </B><BR/>Mouse Anti-RAVER1 Clone: RAV1 (<B>Cat. No. SAB4200203</B>), was used to immunoprecipitate RAVER1 from 293T whole cell lysate. Detection antibody: Rabbit Anti-Raver1 (N-terminal) (<B>Cat. No. SAB4200091</B>) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (<B>Cat. No. R3155</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Negative control: without antibody<BR/>2. 10 μg antibody<BR/>3. 20 μg antibody<BR/>4. Positive control: 293T whole cell lysate

Immunoprecipitation
Mouse Anti-RAVER1 Clone: RAV1 (Cat. No. SAB4200203), was used to immunoprecipitate RAVER1 from 293T whole cell lysate. Detection antibody: Rabbit Anti-Raver1 (N-terminal) (Cat. No. SAB4200091) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (Cat. No. R3155) and a chemiluminescent substrate.
Lanes
1. Negative control: without antibody
2. 10 μg antibody
3. 20 μg antibody
4. Positive control: 293T whole cell lysate