Anti-NEZHA (C-terminal) antibody produced in rabbit

Name	Anti-NEZHA (C-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200171
Prices	$322.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to a sequence at the C-terminal region of human NEZHA conjugated to KLH
Description	Rabbit Polyclonal
Gene	CAMSAP3
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B> <BR/>Staining of Nezha in MDCK cells. Cells were fixed and stained with 2.5 μg/mL Rabbit Anti-Nezha (C-terminal) (<B>Cat. No. SAB4200171</B>) followed by Goat Anti-Rabbit, Cy3 conjugate (×40 magnification).

Immunofluorescence
Staining of Nezha in MDCK cells. Cells were fixed and stained with 2.5 μg/mL Rabbit Anti-Nezha (C-terminal) (Cat. No. SAB4200171) followed by Goat Anti-Rabbit, Cy3 conjugate (×40 magnification).

<B>Immunoblotting</B> <BR/>Lysate of HEK-293T cells over-expressing Nezha was separated on SDS-PAGE and probed with Rabbit Anti-Nezha (C-Terminal) (<B>Cat. No. SAB4200171</B>). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (<B>Cat. No. A0545</B>) and a chemiluminescence substrate. <BR/><B>Lanes</B><BR/>1. Antibody: 2 μg/mL<BR/>2. Antibody: 1 μg/mL<BR/>3. Antibody: 0.5 μg/mL<BR/>4. Antibody: 0.25 μg/mL<BR/>5. Antibody: 1 μg/mL + 10 μg/mL Nezha immunizing peptide

Immunoblotting
Lysate of HEK-293T cells over-expressing Nezha was separated on SDS-PAGE and probed with Rabbit Anti-Nezha (C-Terminal) (Cat. No. SAB4200171). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate.
Lanes
1. Antibody: 2 μg/mL
2. Antibody: 1 μg/mL
3. Antibody: 0.5 μg/mL
4. Antibody: 0.25 μg/mL
5. Antibody: 1 μg/mL + 10 μg/mL Nezha immunizing peptide

<B>Immunoprecipitation</B><BR/>Rabbit Anti-Nezha (C-terminal) (<B>Cat. No. SAB4200171</B>) was used to immunoprecipitate human Nezha from lysate of HEK-293T cells over-expressing human Nezha. Detection Antibody Rabbit Anti-Nezha (N-terminal) (<B>Cat. No. SAB4200172</B>).<BR/><B>Lanes</B><BR/>1. IP antibody: 10 μg <BR/>2. IP antibody: 5 μg <BR/>3. IP antibody: 2.5 μg

Immunoprecipitation
Rabbit Anti-Nezha (C-terminal) (Cat. No. SAB4200171) was used to immunoprecipitate human Nezha from lysate of HEK-293T cells over-expressing human Nezha. Detection Antibody Rabbit Anti-Nezha (N-terminal) (Cat. No. SAB4200172).
Lanes
1. IP antibody: 10 μg
2. IP antibody: 5 μg
3. IP antibody: 2.5 μg