Western blot analysis of GAL4 (DBD 1-147): sc-4050 tagged fusion protein (20 ng). Antibodies tested include GAL4 (DBD) (RK5C1): sc-510 tested at 1.0 µg/ml (A), 0.1 µg/ml (B) and 0.01 µg/ml (C) and GAL4 (DBD): sc-577 tested at 0.1 µg/ml (D) and 0.01 µg/ml (E).