ImmunoblottingLysate of HEK-293T cells overexpressing human FLAG
®-C9orf152 fusion protein was separated on SDS-PAGE, blotted with Anti-C9orf152 (156-170) (
Cat. No. SAB1103692) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:1,000
2. Antibody dilution 1:1,000 + C9orf152 immunizing peptide (human, 156-170)
3. Negative control (only secondary antibody)
4. Anti-FLAG
® antibody (
Cat. No. F7425), working concentration 2 μg/ml.