Anti-COG5 antibody produced in rabbit

Name	Anti-COG5 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200440
Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human, Mouse, Rat
Antigen	peptide corresponding to the C-terminal region of human COG5, conjugated to KLH
Description	Rabbit Polyclonal
Gene	COG5
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 1% paraformaldehyde in a buffer containing 10 mM MES pH 6.2, 10 mM NaCl, 1.5 mM MgCl<SUB>2</SUB> and 2.5% glycerol and subsequently permeabilized with 0.1% Triton<SUP>™</SUP> X-100. Fixed cells were co-immunostained with 2 μg Rabbit Anti-COG5 (<B>Cat. No. SAB4200440</B>) followed by Goat Anti-Rabbit IgG, Cy3<SUP>™</SUP> conjugate (red, panel A and B) and with a Golgi marker (green, panel B). Cells were counterstained with DAPI to stain nuclei (blue, panel B). The pictures are courtesy of Dr. Orly Laufman and Prof. Sima Lev, Dept. of Molecular Cell Biology, Weizmann institute of Science, Rehovot, Israel.

Immunofluorescence
HeLa cells were fixed and permeabilized with 1% paraformaldehyde in a buffer containing 10 mM MES pH 6.2, 10 mM NaCl, 1.5 mM MgCl₂ and 2.5% glycerol and subsequently permeabilized with 0.1% Triton^™ X-100. Fixed cells were co-immunostained with 2 μg Rabbit Anti-COG5 (Cat. No. SAB4200440) followed by Goat Anti-Rabbit IgG, Cy3^™ conjugate (red, panel A and B) and with a Golgi marker (green, panel B). Cells were counterstained with DAPI to stain nuclei (blue, panel B). The pictures are courtesy of Dr. Orly Laufman and Prof. Sima Lev, Dept. of Molecular Cell Biology, Weizmann institute of Science, Rehovot, Israel.

<B>Immunoblotting</B><BR/>Whole extract of HEK-293T cells overexpressing human COG5 cells was separated on SDS-PAGE and probed with Rabbit Anti-COG5 (<B>Cat. No. SAB4200440</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/>Lanes<BR/>1. 2 μg/mL antibody<BR/>2. 4 μg/mL antibody<BR/>3. 4 μg/mL antibody + COG5 immunizing peptide

Immunoblotting
Whole extract of HEK-293T cells overexpressing human COG5 cells was separated on SDS-PAGE and probed with Rabbit Anti-COG5 (Cat. No. SAB4200440). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 4 μg/mL antibody
3. 4 μg/mL antibody + COG5 immunizing peptide