ImmunofluorescenceHepG2 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-PUS7 Clone: PUS7-A187 (
Cat. No. SAB4100173) diluted to 1:100. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingLysate of HEK-293T cells expressing FLAG
®-tagged human PUS7 was separated on SDS-PAGE, probed with Mouse Anti-PUS7 Clone: PUS7-A187 (
Cat. No. SAB4100173). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:2,000
2. Negative control
3. Positive control Anti-FLAG
®
ImmunoblottingHepG2 cell extract was separated on SDS-PAGE and probed with Mouse Anti-PUS7 Clone: PUS7-A187 (
Cat. No. SAB4100173). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:1,000
2. Negative control