ImmunofluorescenceMDA-MB-231 cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Mouse Anti-TBCEL Clone: TBCEL-B209 (
Cat. No. SAB4100284) diluted to 1:25. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingLysate of HEK-293T cells expressing FLAG
®-tagged human TBCEL was separated on SDS-PAGE, probed with Mouse Anti-TBCEL Clone: TBCEL-B209 (
Cat. No. SAB4100284). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:2,000
2. Negative control
3. Positive control Anti-FLAG
®