Anti-ATP1A2 (566-580) antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: A8235
• Prices: $328.50
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: synthetic peptide corresponding to amino acids 566-580 of human ATP1A2
• Description: Rabbit Polyclonal
• Gene: ATP1A2
• Conjugate: unconjugated

Product images

Immunofluorescence
Anti-ATP1A2 (566-580), Cat. No. A8235: Immunofluorescence of HUVEC cells using Anti-ATP1A2 (566-580), A8235 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunoblotting
HEK-293T cell lysate was separated on SDS-PAGE, blotted with Anti-ATP1A2 (566-580) (Cat. No. A8235) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + ATP1A2 immunizing peptide (human, 566-580)

Immunofluorescence Graph
Anti-ATP1A2 (566-580), Cat. No. A8235: Intensity analysis of Anti-ATP1A2 (566-580) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different Antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Haploinsufficiency of ATP1A2 encoding the Na+/K+ pump alpha2 subunit associated - Haploinsufficiency of ATP1A2 encoding the Na+/K+ pump alpha2 subunit associated

De Fusco M, Marconi R, Silvestri L, Atorino L, Rampoldi L, Morgante L, Ballabio A, Aridon P, Casari G. Nat Genet. 2003 Feb;33(2):192-6. Epub 2003 Jan 21.

Ion pumps in polarized cells: sorting and regulation of the Na+, K+- and H+, - Ion pumps in polarized cells: sorting and regulation of the Na+, K+- and H+,

Dunbar LA, Caplan MJ. J Biol Chem. 2001 Aug 10;276(32):29617-20. Epub 2001 Jun 12.