ImmunofluorescenceAnti-AMPD1 (531-545):
Cat. No. A1860: Immunofluorescence of HUVEC cells using AMPD1 (531-545), Cat. No. A1860 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
ImmunoblottingLysate of HEK-293T cells overexpressing human AMPD1 was separated on SDS-PAGE, blotted with Anti-AMPD1 (531-545) (
Cat. No. A1860) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
2. Antibody dilution 1:500 + AMPD1 immunizing peptide (human, 531-545)
3. Antibody dilution 1:1,000
ChartAnti-AMPD1 (531-545):
Cat. No. A1860: Intensity analysis of Anti-AMPD1 (531-545) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.