Anti-DIRAS1 (176-190) antibody produced in rabbit

Name	Anti-DIRAS1 (176-190) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	D1320
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 176-190 of human DIRAS1
Description	Rabbit Polyclonal
Gene	DIRAS1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-DIRAS1 (176-190): <B>Cat. No. D1320</B>: Immunofluorescence of HUVEC cells using DIRAS1 (176-190) (RB),<B>Cat. No. D1320</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-DIRAS1 (176-190): Cat. No. D1320: Immunofluorescence of HUVEC cells using DIRAS1 (176-190) (RB),Cat. No. D1320 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human DIRAS1 was separated on SDS-PAGE, blotted with Anti-DIRAS1 (176-190) (<B>Cat. No. D1320</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + DIRAS1 immunizing peptide (human, 176-190) <BR/>3. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human DIRAS1 was separated on SDS-PAGE, blotted with Anti-DIRAS1 (176-190) (Cat. No. D1320) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + DIRAS1 immunizing peptide (human, 176-190)
3. Antibody dilution 1:1,000

<B>Chart</B><BR/>Anti-DIRAS1 (176-190): <B>Cat. No. D1320</B>: Intensity analysis of Anti-DIRAS1 (176-190) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-DIRAS1 (176-190): Cat. No. D1320: Intensity analysis of Anti-DIRAS1 (176-190) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Di-Ras, a distinct subgroup of ras family GTPases with unique biochemical - Di-Ras, a distinct subgroup of ras family GTPases with unique biochemical

Kontani K, Tada M, Ogawa T, Okai T, Saito K, Araki Y, Katada T. J Biol Chem. 2002 Oct 25;277(43):41070-8. Epub 2002 Aug 22.

Rig is a novel Ras-related protein and potential neural tumor suppressor. - Rig is a novel Ras-related protein and potential neural tumor suppressor.

Ellis CA, Vos MD, Howell H, Vallecorsa T, Fults DW, Clark GJ. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9876-81. Epub 2002 Jul 9.

Downregulation of the novel tumor suppressor DIRAS1 predicts poor prognosis in - Downregulation of the novel tumor suppressor DIRAS1 predicts poor prognosis in

Zhu YH, Fu L, Chen L, Qin YR, Liu H, Xie F, Zeng T, Dong SS, Li J, Li Y, Dai Y, Xie D, Guan XY. Cancer Res. 2013 Apr 1;73(7):2298-309.