Monoclonal Anti-TUBA8 antibody produced in mouse

Name	Monoclonal Anti-TUBA8 antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4100010
Prices	$377.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone	TUBA8-C14
Applications	WB
Species Reactivities	Human
Antigen	TUBA8 purified recombinant protein
Description	Mouse Monoclonal
Gene	TUBA8
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HepG2 cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Mouse Anti-TUBA8 Clone: TUBA8-C14 (<B>Cat. No. SAB4100010</B>) diluted to 1:10. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.

Immunofluorescence
HepG2 cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Mouse Anti-TUBA8 Clone: TUBA8-C14 (Cat. No. SAB4100010) diluted to 1:10. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells expressing FLAG<SUP>®</SUP>-tagged human TUBA8 was separated on SDS-PAGE, probed with Mouse Anti-TUBA8 Clone: TUBA8-C14 (<B>Cat. No. SAB4100010</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:1,000<BR/>3. Negative control<BR/>4. Positive control Anti-FLAG<SUP>®</SUP>

Immunoblotting
Lysate of HEK-293T cells expressing FLAG^®-tagged human TUBA8 was separated on SDS-PAGE, probed with Mouse Anti-TUBA8 Clone: TUBA8-C14 (Cat. No. SAB4100010). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:1,000
3. Negative control
4. Positive control Anti-FLAG^®

<B>Immunoblotting</B><BR/>HepG2 cell extract was separated on SDS-PAGE and probed with Mouse Anti-TUBA8 Clone: TUBA8-C14 (<B>Cat. No. SAB4100010</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:250<BR/>2. Antibody dilution 1:500<BR/>3. Negative control

Immunoblotting
HepG2 cell extract was separated on SDS-PAGE and probed with Mouse Anti-TUBA8 Clone: TUBA8-C14 (Cat. No. SAB4100010). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:250
2. Antibody dilution 1:500
3. Negative control

<B>Flow Cytometry</B><BR/>1×10<SUP>6</SUP> HT29 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton X-100. Fixed cells were labeled with Mouse Anti-TUBA8 Clone: TUBA8-C14 (<B>Cat. No. SAB4100010</B>) diluted to 1:10 followed by Rabbit Anti-Mouse IgG, FITC conjugate. Shaded histogram: negative control without first antibody, Gray histogram: antibody specific staining.

Flow Cytometry
1×10⁶ HT29 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton X-100. Fixed cells were labeled with Mouse Anti-TUBA8 Clone: TUBA8-C14 (Cat. No. SAB4100010) diluted to 1:10 followed by Rabbit Anti-Mouse IgG, FITC conjugate. Shaded histogram: negative control without first antibody, Gray histogram: antibody specific staining.