ImmunofluorescenceHeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2.5 μg/mL Rabbit Anti-AMSH-LP/STAMBPL1 (
Cat. No. SAB4200145). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (
Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingWhole extract of PMC2 cells was separated on SDS-PAGE and probed with Rabbit Anti-AMSH-LP/STAMBPL1 (
Cat. No. SAB4200145). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. 1 μg/mL antibody
2. 2 μg/mL antibody
3. 2 μg/mL antibody + AMSH-LP/STAMBPL1 immunizing peptide