ImmunofluorescenceNuclear localization of HOXD4 in HEK-293T cells over expressing human HOXD4. Cells were fixed and stained with 0.5 μg/mL Rabbit Anti-HOXD4 (C-terminal) (
Cat. No. H3166) followed by Goat Anti-Rabbit, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei (x40 magnification).
ImmunoprecipitationRabbit Anti-HOXD4 (C-terminal) (
Cat. No. H3166) was used to immunoprecipitate HOXD4 from lysate of HEK-293T over expressing human HOXD4. Detection antibody: 1 μg/mL Anti-HOXD4 (N-terminal) (
Cat. No. H3291)
Lanes1. 5 μg antibody
2. 2.5 μg antibody
3. 1.2 μg antibody
4. Negative control without IP antibody
5. Negative control without cell lysate