Anti-GCAT (116-128) antibody produced in rabbit

Name	Anti-GCAT (116-128) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	G6422
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 116-128 of human GCAT
Description	Rabbit Polyclonal
Gene	GCAT
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-GCAT (116-128): <B>Cat. No. G6422</B>: Immunofluorescence of HUVEC cells using GCAT (116-128), Cat. No. G6422 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-GCAT (116-128): Cat. No. G6422: Immunofluorescence of HUVEC cells using GCAT (116-128), Cat. No. G6422 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human GCAT was separated on SDS-PAGE, blotted with Anti-GCAT (116-128) (<B>Cat. No. G6422</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + GCAT immunizing peptide (human, 116-128)

Immunoblotting
Lysate of HEK-293T cells overexpressing human GCAT was separated on SDS-PAGE, blotted with Anti-GCAT (116-128) (Cat. No. G6422) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + GCAT immunizing peptide (human, 116-128)

<B>Chart</B><BR/>Anti-GCAT (116-128): <B>Cat. No. G6422</B>: Intensity analysis of Anti-GCAT (116-128) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-GCAT (116-128): Cat. No. G6422: Intensity analysis of Anti-GCAT (116-128) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Molecular cloning of the human and murine 2-amino-3-ketobutyrate coenzyme A - Molecular cloning of the human and murine 2-amino-3-ketobutyrate coenzyme A

Edgar AJ, Polak JM. Eur J Biochem. 2000 Mar;267(6):1805-12.

Interaction between L-threonine dehydrogenase and aminoacetone synthetase and - Interaction between L-threonine dehydrogenase and aminoacetone synthetase and

Tressel T, Thompson R, Zieske LR, Menendez MI, Davis L. J Biol Chem. 1986 Dec 15;261(35):16428-37.

Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and - Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and

Hoshino A, Fujii H. Cell Stress Chaperones. 2007 Summer;12(2):186-91.