ImmunoblottingWhole extract of HEK-293T cells overexpressing human ATG101 was separated on SDS-PAGE and probed with Monoclonal Anti-ATG101 Clone: ATG101-15 (
Cat. No. SAB4200506). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. 1 μg/mL antibody
2. 0.5 μg/mL antibody
3. Negative control: without 1st antibody
ImmunoprecipitationMonoclonal Anti-ATG101 Clone: ATG101-15 (
Cat. No. SAB4200506) was used to immunoprecipitate ATG101 from HEK-293T cells overexpressing human ATG101 whole cell extract. Detection antibody: Rabbit Anti-ATG101 (
Cat. No. SAB4200175).
Lanes1. Positive control: whole cell extract
2. 10 μg antibody
3. 5 μg antibody