ImmunoblottingLysate of HEK-293T cells was separated on SDS-PAGE, blotted with Anti-PEF1 (21-35) (
Cat. No. SAB1103332) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
ImmunoblottingLysate of HEK-293T cells overexpressing human PEF1 was separated on SDS-PAGE, blotted with Anti-PEF1 (21-35) (
Cat. No. SAB1103332) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:1,000
2. Antibody dilution 1:1,000 + PEF1 immunizing peptide (human, 21-35)