Monoclonal Anti-ZFYVE28/LST2 antibody produced in mouse

Name	Monoclonal Anti-ZFYVE28/LST2 antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4200419
Prices	$337.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone	LST139
Applications	ICC/IF WB
Species Reactivities	Human
Antigen	peptide corresponding to the N-terminus of human ZFYVE28 /LST2, conjugated to KLH
Description	Mouse Monoclonal
Gene	ZFYVE28
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>MCF-7 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 0.25 μg/mL Mouse Anti-ZFYVE28/LST2 Clone: LST139 (<B>Cat. No. SAB4200419</B>). The antibody was developed using Goat Anti-Mouse IgG, CY3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
MCF-7 cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 0.25 μg/mL Mouse Anti-ZFYVE28/LST2 Clone: LST139 (Cat. No. SAB4200419). The antibody was developed using Goat Anti-Mouse IgG, CY3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Whole extract of MCF-7 cells was separated on SDS-PAGE and probed with Mouse Anti-ZFYVE28/LST2 Clone: LST139 (<B>Cat. No. SAB4200419</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. 16 μg/mL antibody<BR/>2. 8 μg/mL antibody <BR/>3. 4 μg/mL antibody <BR/>4. 2 μg/mL antibody<BR/>5. Negative control

Immunoblotting
Whole extract of MCF-7 cells was separated on SDS-PAGE and probed with Mouse Anti-ZFYVE28/LST2 Clone: LST139 (Cat. No. SAB4200419). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. 16 μg/mL antibody
2. 8 μg/mL antibody
3. 4 μg/mL antibody
4. 2 μg/mL antibody
5. Negative control

<B>Immunoblotting</B><BR/>Lysates were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-ZFYVE28/LST2 Clone: LST139 (<B>Cat. No. SAB4200419</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. MCF-7; 2. 293T; 3. MDBK; 4. MDCK; 5. P19; 6. COS-7; 7. NRK

Immunoblotting
Lysates were separated on SDS-PAGE and probed with 2 μg/mL Mouse Anti-ZFYVE28/LST2 Clone: LST139 (Cat. No. SAB4200419). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. MCF-7; 2. 293T; 3. MDBK; 4. MDCK; 5. P19; 6. COS-7; 7. NRK