ImmunofluorescenceAnti-UBL5 (51-65):
Cat. No. U5009: Immunofluorescence of HUVEC cells using UBL5 (51-65) (RB),
Cat. No. U5009 (red) at a 1:200 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
ImmunoblottingLysate of HEK-293T cells overexpressing human UBL5 was separated on SDS-PAGE, blotted with Anti-UBL5 (51-65) (
Cat. No. U5009) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:250
ChartAnti-UBL5 (51-65):
Cat. No. U5009: Intensity analysis of Anti-UBL5 (51-65) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.