ImmunoblottingLysate of HEK-293T cells overexpressing human MSTN was separated on SDS-PAGE, blotted with Anti-MSTN (191-205) (
Cat. No. M3448) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:250
ImmunoblottingLysate of HEK-293T cells overexpressing human RPS6KC1 was separated on SDS-PAGE, blotted with Anti-RPS6KC1 (756-770) (
Cat. No. R9655) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
2. Antibody dilution 1:500 + RPS6KC1 immunizing peptide (human, 756-770)
3. Antibody dilution 1:1,000