Anti-GPR137B (13-25) antibody produced in rabbit

Name	Anti-GPR137B (13-25) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	G1422
Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 13-25 of human GPR137B
Description	Rabbit Polyclonal
Gene	GPR137B
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-GPR137B (13-25) (RB): <B>Cat. No. G1422</B>: Immunofluorescence of HUVEC cells using GPR137B (13-25) (RB), <B>Cat. No. G1422</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.<BR/>Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-GPR137B (13-25) (RB): Cat. No. G1422: Immunofluorescence of HUVEC cells using GPR137B (13-25) (RB), Cat. No. G1422 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human GPR137B was separated on SDS-PAGE, blotted with Anti-GPR137B (13-25) (<B>Cat. No. G1422</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human GPR137B was separated on SDS-PAGE, blotted with Anti-GPR137B (13-25) (Cat. No. G1422) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:1,000

<B>Chart</B><BR/>Anti-GPR137B (13-25) (RB): <B>Cat. No. G1422</B>: Intensity analysis of Anti-GPR137B (13-25) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-GPR137B (13-25) (RB): Cat. No. G1422: Intensity analysis of Anti-GPR137B (13-25) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Cloning and characterization of a novel gene (TM7SF1) encoding a putative - Cloning and characterization of a novel gene (TM7SF1) encoding a putative

Spangenberg C, Winterpacht A, Zabel BU, Lobbert RW. Genomics. 1998 Mar 1;48(2):178-85.

TM7SF1 (GPR137B): a novel lysosome integral membrane protein. - TM7SF1 (GPR137B): a novel lysosome integral membrane protein.

Gao J, Xia L, Lu M, Zhang B, Chen Y, Xu R, Wang L. Mol Biol Rep. 2012 Sep;39(9):8883-9.

Expression and characterization of a 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid - Expression and characterization of a 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid

Jones CE, Holden S, Tenaillon L, Bhatia U, Seuwen K, Tranter P, Turner J, Kettle R, Bouhelal R, Charlton S, Nirmala NR, Jarai G, Finan P. Mol Pharmacol. 2003 Mar;63(3):471-7.

Identification of a novel human eicosanoid receptor coupled to G(i/o). - Identification of a novel human eicosanoid receptor coupled to G(i/o).

Hosoi T, Koguchi Y, Sugikawa E, Chikada A, Ogawa K, Tsuda N, Suto N, Tsunoda S, Taniguchi T, Ohnuki T. J Biol Chem. 2002 Aug 30;277(35):31459-65. Epub 2002 Jun 13.