Anti-VGLL4 (53-65) antibody produced in rabbit

Name	Anti-VGLL4 (53-65) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	V2765
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 53-65 of human VGLL4
Description	Rabbit Polyclonal
Gene	VGLL4
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human FLAG- VGLL4 fusion protein was separated on SDS-PAGE, blotted with Anti-VGLL4 (53-65) (<B>Cat. No. V2765</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + VGLL4 immunizing peptide (human, 53-65)<BR/>3. Antibody dilution 1:1,000<BR/>4. Negative control (only secondary antibody)<BR/>5. Anti-FLAG antibody (<B>Cat. No. F7425</B>), working concentration 2 μg/mL

Immunoblotting
Lysate of HEK-293T cells overexpressing human FLAG- VGLL4 fusion protein was separated on SDS-PAGE, blotted with Anti-VGLL4 (53-65) (Cat. No. V2765) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + VGLL4 immunizing peptide (human, 53-65)
3. Antibody dilution 1:1,000
4. Negative control (only secondary antibody)
5. Anti-FLAG antibody (Cat. No. F7425), working concentration 2 μg/mL

<B>Immunofluorescence</B><BR/>Anti-VGLL4 (53-65): <B>Cat. No. V2765</B>: Immunofluorescence of HUVEC cells using VGLL4 (53-65), Cat. No. V2765 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-VGLL4 (53-65): Cat. No. V2765: Immunofluorescence of HUVEC cells using VGLL4 (53-65), Cat. No. V2765 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Chart</B><BR/>Anti-VGLL4 (53-65): <B>Cat. No. V2765</B>: Intensity analysis of Anti-VGLL4 (53-65) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-VGLL4 (53-65): Cat. No. V2765: Intensity analysis of Anti-VGLL4 (53-65) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.