ImmunofluorescenceA431 cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 2.5 μg/mL Monoclonal Anti-WDR37, Clone: WDR37-9 (
Cat. No. SAB4200464). The antibody was developed using Goat Anti-Mouse IgG, Cy3
™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingCell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Monoclonal Anti-WDR37, Clone: WDR37-9 (
Cat. No. SAB4200464). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. MDCK
2. MDBK
3. U87