Anti-IFITM5 Antibody (Center) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-IFITM5 Antibody (Center) at 1:4000 dilution Lane 1: T47D whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: SW480 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: PANC-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-IFITM5 Antibody (Center) at 1:2000 dilution Lane 1: PC-3 whole cell lysate Lane 2: SW480 whole cell lysate Lane 3: HT-29 whole cell lysate Lane 4: Mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.