Western blot analysis of lysates from 293,K562,PC-3 cell line (from left to right),using PIP5KL1 Antibody (N-term)(Cat. #AP11297a).AP11297a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
PIP5KL1 Antibody (N-term) (Cat. #AP11297a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIP5KL1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of PIP5KL1 Antibody (N-term)(Cat#AP11297a) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).