SDHD Antibody (N-term) (Cat. #AP12155a) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SDHD antibody detected the SDHD protein (arrow).
SDHD Antibody (N-term) (Cat. #AP12155a)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SDHD Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of SDHD Antibody (N-term)(Cat#AP12155a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
SDHD Antibody (N-term) (Cat. #AP12155a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-SDHD Antibody (N-term) at 1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.