PURB-Antibody-C-term
| Name | PURB-Antibody-C-term |
|---|---|
| Supplier | Abgent, a WuXi AppTec company |
| Catalog | AP12051b |
| Prices | $95.00, $265.00 |
| Sizes | 80 µl, 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Applications | WB ICC/IF FC ELISA |
| Species Reactivities | Human |
| Antigen | 260-289 aa |
| Purity/Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Description | Rabbit Polyclonal |
| Gene | PURB |
| Supplier Page | Shop |
Product images
Fluorescent image of A549 cell stained with PURB Antibody (C-term)(Cat#AP12051b).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PURB primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).PURB immunoreactivity is localized to Nucleus significantly.
PURB Antibody (C-term) (Cat. #AP12051b) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the PURB antibody detected the PURB protein (arrow).
PURB Antibody (C-term) (Cat. #AP12051b) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.