SLC16A10 Antibody (C-term) (Cat. #AP13235b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SLC16A10 antibody detected the SLC16A10 protein (arrow).
SLC16A10 Antibody (C-term) (Cat. #AP13235b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SLC16A10 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
SLC16A10 Antibody (C-term) (Cat. #AP13235b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-SLC16A10 Antibody (C-term) at 1:2000 dilution Lane 1: human skeletal muscle lysates Lane 2: CCRF-CEM whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP13235b staining SLC16A10 in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.